| Acronym | Definition |
|---|
| DEF | Definition |
| DEF | Definite |
| DEF | Defrost |
| DEF | Diesel Exhaust Fluid (automotive) |
| DEF | Definitions (File Name Extension) |
| DEF | Defend(ed) |
| DEF | Deftones (band) |
| DEF | Defense(ive) |
| DEF | Defaults (File Name Extension) |
| DEF | Documento di Economia e Finanza (Italian: Economic and Financial Document) |
| DEF | Diplôme d'Etudes Fondamentales (French: Fundamental Studies Diploma) |
| DEF | Détection Électronique Française (French: French Electronic Detection) |
| DEF | Diplôme d'Etudes Françaises (French: Diploma of French Studies; various universities) |
| DEF | Dark Elf (Everquest) |
| DEF | Diamond Empowerment Fund |
| DEF | Diccionario de Especialidades Farmaceuticas (Spanish: Dictionary of Specialty Pharmaceuticals; software) |
| DEF | Digital Empowerment Foundation (New Delhi, India) |
| DEF | Default |
| DEF | Data Element Framework |
| DEF | Discovery Eye Foundation (nonprofit for eye disease research; California) |
| DEF | Developmental Evaluation Framework |
| DEF | Danmarks Elektroniske Forskningsbibliotek |
| DEF | Data Exchange Format |
| DEF | Delayed Ettringite Formation (concrete) |
| DEF | Design Exchange Format (Cadence) |
| DEF | Diplôme d'Entraineur Fédéral de Football (French: Federal Football Coach Diploma) |
| DEF | Decision Education Foundation |
| DEF | Disarmed Enemy Forces |
| DEF | Development Education Forum |
| DEF | Defense Enterprise Fund |
| DEF | Département de l'Emploi Francophone (French: Department of Employment Francophone; various locations) |
| DEF | Drug Education Forum |
| DEF | Deployment Expeditionary Force (US Army) |
| DEF | Detector and Extractor of Fileprints |
| DEF | Direct Equipment Failure |
| DEF | Decayed, Extracted, and Filled Tooth |
| DEF | Dose Effect Factor |
| DEF | Digital Elimination Force |
| DEF | Development & Evaluation Facility |
| DEF | Distance Enumerator Function |
| DEF | Digital Enforcement Faction |
| DEF | Dependents Function |
| DEF | Detection Efficiency Function (microscope) |
| DEF | Defensman/Defensmen (hockey) |