| Acronym | Definition |
|---|
| AFL | American Federation of Labor |
| AFL | American Football League (1960s) |
| AFL | Australian Football League |
| AFL | Arena Football League |
| AFL | Alberta Federation of Labour |
| AFL | All for Less (phone service plan) |
| AFL | Assessment for Learning (education) |
| AFL | Afloat |
| AFL | Adaptive Forward Lighting (automotive engineering) |
| AFL | Academic Free License (software license) |
| AFL | America Fujikura Ltd. (various locations) |
| AFL | Aeroflot (Russian Airline, ICAO code) |
| AFL | Armed Forces of Liberia |
| AFL | American Fuzzy Lop (rabbit) |
| AFL | Active for Life (various organizations) |
| AFL | Atrial Flutter |
| AFL | Accel Frontline Ltd. (India) |
| AFL | Arabic as a Foreign Language (various locations) |
| AFL | Academic Free License |
| AFL | Abstract Family of Language |
| AFL | Adaptive Forward Lighting |
| AFL | Association Française pour la Lecture (French: French Reading Association) |
| AFL | Advance Fee Loan (various locations) |
| AFL | Association Française du Lupus (French: French Lupus Association) |
| AFL | Association Française de Limnologie (French: French Limnology Association) |
| AFL | Air Fluid Level |
| AFL | Alcoholics for Life |
| AFL | Alcoholic Fatty Liver |
| AFL | Abstract Family of Languages |
| AFL | Association Familiale du Limousin (French: Limousin Family Association; Limousin, France) |
| AFL | Acemai France Logique (French elevator manufacturer) |
| AFL | Above Floor Level |
| AFL | Artists for Literacy (San Francisco, CA) |
| AFL | After Fader Listening (audio mixing) |
| AFL | Autofocus Lock (cameras) |
| AFL | Assisted Family Living (various locations) |
| AFL | Air Force Letter |
| AFL | AbsoFreakinLutely |
| AFL | Authorized Funding Level |
| AFL | Airborne Flying Laboratory |
| AFL | Active Fuel Length |
| AFL | Autoland Flight Tests |
| AFL | Approximate Full Load |
| AFL | Automatic Line Feed |
| AFL | Applied Fisheries Laboratory |
| AFL | Auxiliary Fuel Line |
| AFL | Away From Link |