| Acronym | Definition |
|---|
| AFI | American Film Institute |
| AFI | Awaiting Further Instructions |
| AFI | A Fire Inside (band) |
| AFI | Air Force Instruction |
| AFI | Australian Film Institute |
| AFI | Alianza Para la Familia Integral (Spanish: Alliance for the Integral Family) |
| AFI | Agencia Federal de Investigacion (Mexico) |
| AFI | Akademi Fantasi Indosiar (Indonesian reality TV) |
| AFI | Agencia Federal de Investigación (Spanish) |
| AFI | Air France Industries |
| AFI | Agence Française Informatique (French: French Information Agency) |
| AFI | Air Force of India |
| AFI | Address Family Identifier |
| AFI | Armed Forces Insurance |
| AFI | Amniotic Fluid Index |
| AFI | Assets for Independence (Washington, DC) |
| AFI | American Fiberbodies International |
| AFI | Authority and Format Identifier |
| AFI | American Fitness Index |
| AFI | Arla Foods Ingredients |
| AFI | American Family Insurance |
| AFI | Adjusted for Inflation |
| AFI | A Friend Indeed |
| AFI | Asking for It (gaming clan) |
| AFI | Asking for It (band) |
| AFI | Air Force Installation |
| AFI | Association of Food Industries (Neptune, New Jersey) |
| AFI | Adult Film Industry |
| AFI | Art Fairs International |
| AFI | Année Francophone Internationale (French: International Francophone Year) |
| AFI | Application Family Identifier |
| AFI | Applied Finance and Investment (graduate studies) |
| AFI | Alternative Financial Institution |
| AFI | Alfabeto Fonético Internacional (International Phonetic Association) |
| AFI | Aquarium Fish International (monthly magazine) |
| AFI | Available For Inspection |
| AFI | Approved Financial Institution |
| AFI | Advanced Flight Instructor (aviation) |
| AFI | Authority and Format Indicator |
| AFI | Area for Improvement |
| AFI | Authority & Format Identifier |
| AFI | Accountancy, Finance & Insurance |
| AFI | American Football in Israel |
| AFI | Associazione Farmaceutici dell'Industria |
| AFI | Accredited Financial Institution |
| AFI | American Firearms Industry |
| AFI | Aquatic Fitness Instructor |
| AFI | Australian Foundry Institute |
| AFI | Authority Frame Identifier |
| AFI | Asociación Fonética Internacional |
| AFI | Aruba Florin (currency) |
| AFI | Anthems for Insubordinates (band) |
| AFI | Allied Forces Italy |
| AFI | Ah, Forget It (polite form) |
| AFI | Alan Franken, Inc. |
| AFI | American Forest Institute |
| AFI | Abstract Functional Interface |
| AFI | Air Filter Institute |
| AFI | Air Forces Iceland |
| AFI | Australian Financial Institution (classification; Income Tax Assessment Act of 1936; Australia) |
| AFI | AppleTalk Filing Interface |
| AFI | Affinity Index (advertising) |
| AFI | Automatic Fault Isolation |
| AFI | Always False Instruction (programmable logic controllers) |
| AFI | Al-Risala Forum International |
| AFI | African Indian Ocean Geographic Region |
| AFI | After Flight Inspection |
| AFI | Apostles Fellowship International (Christian organization) |
| AFI | Anglicans Friends of Israel |
| AFI | Advantest Finance, Inc. (Japan) |
| AFI | Africa Flight Information (region) |
| AFI | American Fidelity, Inc. |
| AFI | Advanced Financial Integrator |
| AFI | Affordable Fuel Injection (Elsie, MI) |
| AFI | Asahi Fiber Industry (est. 1949; Japan) |
| AFI | Air-to-Fuel Influence |
| AFI | Aircraft Friction Index (braking) |
| AFI | American Forces Italy |
| AFI | Average Failure Intensity |
| AFI | Athens Furniture Industries, Inc (Athens, Tennessee) |
| AFI | Adviser Fund Index (UK) |
| AFI | Applied Futures Inc. |